Sorry to ask but how can I produce VCF_v4.1 after downloading it form here because this folder does not contain any gatk file.
Dear all,
I want to prepare vcf file of 4.1 version. But GATK tool version 4 and above produces vcf version 4.2. Can anyone please share the link of GATK tool's version 3.8 or less. Any hep would be highly appreciated.
Thanks in advance
1 answer
EDIT:
It looks like a bit of a complicated process to install GATK3.8. Here is how you need to do it:
- Install gatk3.8 through conda:
conda create -n gatk38 -c bioconda gatkthen activate the env usingconda activate gatk38 - Get the JAR file from their Google cloud archive location (Exact file: https://storage.googleapis.com/gatk-software/package-archive/gatk/GenomeAnalysisTK-3.8-1-0-gf15c1c3ef.tar.bz2) using
wget https://storage.googleapis.com/gatk-software/package-archive/gatk/GenomeAnalysisTK-3.8-1-0-gf15c1c3ef.tar.bz2 - Register the JAR using
gatk3-register /path/to/GenomeAnalysisTK-3.8-1-0-gf15c1c3ef.tar.bz2. If you did not change directories in any step, you'll basically rungatk3-register GenomeAnalysisTK-3.8-1-0-gf15c1c3ef.tar.bz2. - You're all set. Run
gatk3 --helpto ensure gatk3 works.
Did you try searching for "GATK 3.8" on Google? The fifth result I saw was: https://github.com/broadgsa/gatk
I'm sorry, I don't understand your question.
I mean I am unable to find any gatk script from this link https://github.com/broadgsa/gatk which you send me earlier. If it has no gatk script then what is the command to generate vcf file of version 4.1 from this.
Thanks
That's the github page. You should look under releases to find software that's ready for use. Here is the 3.8-1 release, for example.
Also, you can always install gatk 3.x using conda. conda install -c bioconda gatk
EDIT: Go with the conda solution, the GitHub source code route is a pain
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