MGI Sequencing - Best Script for fixing FASTQ file headers coming from MGI Sequencer's
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
Fixing MGI Sequence Headers - FASTQ Format
written by joe_genome •I am trying to fix the headers of the fastq data from MGI, this comes in a different format than expected Illumina data, this gives …
-
DOES MGI SEQUENCING RESULT CONTAINS MULTIPLE FILES FOR SINGLE SAMPLE
written by manaswiniparija3 •HII.. I got a whole genome sequenced by MGI (short read sequencing). but instead of 2 files(read1 and read2) we got 4 different set of …
-
whole genome sequencing and assembly
written by manaswiniparija3 •we extracted the whole genome of a fish and went for short-read sequencing using an MGI sequencer. usually, when we get sequencing results we get …
-
How to combine and convert fastq PE files into fasta files as batch?
written by sunnykevin97HI I had ~ 100 PE fastq files, I wish to convert them into fasta file separately. (ex. R1 , R2 ---> fasta file, for …
-
Pathogenic predictors for Drosophila variants?
written by darink •Coming from a human genetics background, I have been spoiled for all the ready made tools I have to feed a list of variants into …
-
Multifasta to Singlefasta (replace headers for 'NNNNNNNNNN', then join the multiple contigs)
written by oseias.rf.junior •Hi to all, I'm trying to use a script to find IS on my genomes. But the script will run only on singlefasta files (i.e., …
-
bam to fasta error using samtools
written by ccaggI am trying to convert a bam file to a fasta file, in order to extract a specific fasta region using a bed file. However, …
-
Combining two fasta sequences into one
written by Lille My •I have two fasta files, with the same headers/names for the sequences but different sequences. I would like to combine them into one file, so …
-
If the index sequences in my Illumina FASTQ headers aren't the barcodes, then what are they?
written by jenn.drummondHi, everybody. I have FASTQ headers of the form @FCC3KD2ACXX:6:1101:1545:2184#ATCACGATC/1 The "ATCACGATC" portion of these older-style headers is supposed to be the "index sequence", or …
-
Variant Analysis on MiSeq Data
written by gkuffel22 •Hi everyone, I am trying to figure out the most efficient method to perform variant analysis on a large dataset. I have 200 samples and …
Curious as to why they need "fixing"? Can you show examples of what needs to be fixed.
Can I use them directly for alignment after QC, and recommendations for Alignment scripts that take these FASTQ files, I'm just very new to their analysis? I used to QC FastQ from Illumina and use for Botwei alignment for example, and I'm not sure if I may need to fix headers for MGI FASTQ files .. can you offer me an advice?
If you were able to use FastQC and bowtie for alignment then no "fixing" should be needed for MGI headers. Since MGI seq is not common in US show us examples of what the headers look like.