This worked quite well, thank you
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joe_genome •
Fixing MGI Sequence Headers - FASTQ Format
I am trying to fix the headers of the fastq data from MGI, this comes in a different format than expected Illumina data, this gives issues downstream when trying to use tools such as samtools, hisat2.
@E250087456L2C221R00100000555#GGGTTTA/1
UMIs also come in a separate file so we need to parse this, not sure on how to approach.
Possible approach?
@E250087456L2C221R00100000555#GGGTTTA:AATTGGCCTTAA/1
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joe_genome •
1 answer
Give this a try: https://sagc-bioinformatics.github.io/mgikit/reformat
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