This is a test version of Biostars. For the public version, visit https://www.biostars.org.
How should I interpret FB_Plot in microarray analysis?

Hi,

I have a foreground-background plot using limma plotFB function for one array. I couldn't find any detailed resource of how to interpret this plot.

So what does the plot shows? what is the blue line here? enter image description here

microarray analysis control quality

Do not delete posts that have received feedback. Interact with the people that have invested effort into helping you, and work towards providing closure to your post.

1 answer

The documentation (?plotFB) says:

A foreground-background plot is a plot of log2-foreground vs log2-background for a particular array.

Each dot presents one point on the microarray.

The blue line is simply the line with x=y. The foreground should be equal to background plus signal, so the x=y line estimates the lower bound for the expected foreground values.

Log in to answer this question.