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differential expression analysis

Hello! How to perform differential expression analysis of RT-PCR data for two conditions say control and treatment?

rt-pcr

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1 answer

The deltaDeltaCt method is most common. It's usually appropriate to conduct statistics on deltaCt values, and present RQ (2^-ddCt). Google more for yourself, or see

https://toptipbio.com/delta-delta-ct-pcr/

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