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DEG analysis between control and treatment groups in integrated scRNAseq analysis

I am using the Seurat platform to perform integrated analysis on my single cell data. I have four samples derived from one biological replicate. I have two time points, and for each time point, there is one control and one treatment sample. Can I still perform differential gene expression to compare between conditions/samples within a cluster?

Thank you.

scrnaseq integration deg

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