This is a test version of Biostars. For the public version, visit https://www.biostars.org.
Mate-pair sequencing

Hello!

I'm learning about mate-pair sequencing and I don't really understand how does the process work. I understand the whole procedure of getting only the sequences that have the former DNA ends, but what happens next? What is the advantage of having a sequence built of fragments which were originally separated by several kilobases?

Cheers, Michał

sequecing sequencing mate-pair
• 1,368 views • link
updated by benformatics benformatics • written by Michał • Michał

1 answer

If you don't have a genome assembly... the mate pairs would help you in assembling a non-mate pair library by providing information about long-distance interactions. Any mate pairs that are sequenced are likely intra-chromosomal.

Also in situations with repeats you would get better resolution:

home.cc.umanitoba.ca - mate pairs

• 0 views • link
written by benformatics benformatics

Log in to answer this question.

More posts like this