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small RNA sequencing

Hello everyone,

I have paired end small RNA seq raw data. I have to differential expression analysis. Can anyone help me how to start it?

Thanks

smallrna sequencing

A simple search in google would recommend you mirdeep2. So do I. However, my question is, since mature small RNAs have a mean length of 22 nucleotides, how is it possible to get paired-end reads? What insert size did you use? Did you mean small RNA precursors rather than mature RNAs?

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