interpreting SnpEff output
Hi all.
I ran GATK germline variant discovery on my WES samples.
By running SnpEff on VCF files, many intron variants have been reported.
Why?
Because my sequences are related to exon regions, I expected to see no intron variants in the SnpEFF output.
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1 answer
It could be because of capture method If it were Agilent sureselect Chemistry, V8 + NCV or 5' UTR. Or even the previous versions entails subtle noncoding regions
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