Tool: split pooled paired-end fastq using fqkit
fqkit barcode -h
split barcode for PE reads
Usage: fqkit barcode [OPTIONS] --read1 <READ1> --read2 <READ2> --barcode <BAR>
Options:
-1, --read1 <READ1> input read1 fastq[.gz] file
-2, --read2 <READ2> input read2 fastq[.gz] file, barcode in this file
-b, --barcode <BAR> barcode list file, eg: barcode sample
-m, --mode <MODE> barcode position mode, 1:left, 2:right [default: 2]
-r, --rev_comp barcode reverse complement
-e, --error <MISMATCH> barcode mismatch base count [default: 0]
-o, --outdir <OUTDIR> fastq file output dir [default: .]
-h, --help Print help information
see here: https://github.com/sharkLoc/fqkit
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