Comparing mapped reads between long-read bulk RNA-seq samples
Hi,
I want to compare the count of transcripts between samples. In my experiment, I performed long-read bulk RNA-seq (not PCR amplified) on samples containing plasma cells. We mapped the read to the immunoglobulin database with minimap, which gives us the count of transcripts mapped to the different immunoglobulin. Currently, I am just comparing the TPM between samples. Is this the correct way of comparing samples, or do I need to normalize the data first?
Any help is appreciated
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