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Is there a possibility of Cd45- immune cells in single cell-rna seq?

I have been getting clusters that are expressing lineage markers such as: Cd19, Cd3, Cd4, Igha, Jchain.. etc. but those clusters did not have any Ptprc/ cd45 expression. Its 0. I was not able to find any papers including that type of situation. Do you have any suggestions about that? Are they immune cells? It is important to mention that I am working with cancerous cell population.

Thanks!

single-cell immune-cells scrna-seq
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written by eaglesfeet • eaglesfeet

Could you be seeing something like this?

Flow cytometry analysis of the bone marrow aspirate showed a small monoclonal B-cell population expressing CD10, CD19, CD20, CD22, CD79b, and surface immunoglobulin κ but lacking CD45 (panels E-H). The CD45 antigen is normally universally expressed in variable intensities on all leukocytes. Usually, this marker is used to gate out non-nucleated cells. In this case, however, the CD45-negative follicular lymphoma cells were almost overlooked using the normal gating strategy.

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written by Jesse • Jesse

might be, this is so insightful thanks a lot.

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written by eaglesfeet • eaglesfeet

Its a ptprc - dataset so there is 0 expression of it. Here is a dotplot from my UMAP so the markers are clear. Dotplot of my clusters

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written by eaglesfeet • eaglesfeet

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