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How am I supposed to perform differential expression with .cel files ?

I want to perform differential analysis on GSE90603_RAW which contains miRNA microarray data, but I'm not sure how it's done with .cel files

differential mirna
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updated by GenoMax GenoMax • written by Bhanu • Bhanu

Thanks but what am I supposed to do after normalization?

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written by Bhanu • Bhanu

1 answer

The platform of your array is GPL21572, so after normalization, your measured probe set intensities are proportional to the miRNA quantities in your sample. Subsequently, you can perform differential microarray expression like you would do that for genes. Also mind that there are dedicated miRNA packages for in-depth analysis.

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updated by GenoMax GenoMax • written by Matthias Zepper Matthias Zepper

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