Hi all, Is there a way to download a realistic rna-seq data generated from the reference transcript (https://www.ncbi.nlm.nih.gov/projects/genome/guide/human/index.shtml#:~:text=gff3-,RefSeq%20Transcripts,-Fasta) in SRA? If so, is there an …

Hi biostars, I want to generate a histogram of reference transcript in here (https://www.ncbi.nlm.nih.gov/projects/genome/guide/human/index.shtml#:~:text=gff3-,RefSeq%20Transcripts,-Fasta). Can anyone suggest a tool that can generate a histogram of …

Hi Biostars, I am interested in generating realistic rna-seq. Does anyone know tools that use 1) reference transcript and 2) isoform expression as input and …

Hi All, I wonder if someone can help me in explaining what to input on the `-R <bed file>` argument of the code below? computeMatrix …

Hello Everyone, I am quantifying read counts in the bam using feutureCount in the command line but getting errors below ERROR: failed to find the …

I aligned all my Nanopore Rna-Seq reads with Human Reference Genome and Transcript (Then I had 2 different bam files one of them is aligned …

Hello, I have downloaded the reference for alignment of RNA-Seq with human transcriptome form[This][1] link. I downloaded RefSeq transcripts from the link to use as …

Hello, I have downloaded human transcriptome (RefSeq transcripts) from [this][1] website. I want to download gene annotation file for this transcriptome. Can some one help …

Hello, I have downloaded human transcriptome(RefSeq Transcripts) from [THIS][1] link. I want to annotation file for same which I can use in STAR aligner. Can …

Dear BioStars Leaders, I was under the impression that the nucleotide sequence of an mRNA from Refseq would match identically to the Reference Genome assembly. …