Low number of nCount_RNA in single-cell RNAseq clusters?
Hello, after standard Seurat workflow, I checked the metrics of each cluster in the object. Please see the attached picture. How may I explain, in some of clusters, such as cluster 9 which has low nCount_RNA and nFeature_RNA but with an acceptable percent of mitochondria genes? I would think cluster 9 is a low quality of cell population and may be removed from the clusters? Any suggestions?
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