How can I get polyA position and coverage from polyA(+)RNASeq data?
Hi everyone!
As polyA(+) RNAseq library produce reads with polyA tails, is there any way I can extract the position and coverage informations of reads that contain polyA from mapped sam/bam file?
Thank you!
• 992 views
•
link
1 answer
Just grep the FASTQ for 'AAAAAAA$' and pull the read names. There is no way that the reads aligned without soft-clipping anyway.
• 0 views
•
link
Log in to answer this question.