How can the reads be assemble to unitigs with ONLY EXACT sequence overlaps?
I'm now working on a metagenome assembly project. There, I want to segregate and assemble all of the reads with stringent parameters: they should not overlap segments even if they have a single mutation in the sequence. Is there any software/tools or flags that can assist me in accomplishing this...
Note: At the moment I don't care about N50.
1 answer
Not 100% sure, but probably unitigs of BCALM are what you need (https://github.com/GATB/bcalm)
Log in to answer this question.
More posts like this
-
Package for transcript assembly of reference-aligned ion torrent reads
written by cjwwfd •
-
How to handle multiple contig outputs from de novo assemblers when one contig is desired?
written by Trombone Engineer •
-
Detecting Chimeric Contigs
written by KSS •
-
Mapping soft for de novo assembly
written by takoyaki
-
merging a number of overlapping sanger sequences
written by thomas.welch •
-
Non-cancer somatic mutation calling
written by DVA
-
How can we have SAM / BAM reads to validate a de novo genome assembly?
written by alecloic •
-
Forum: Open Source projects to contribute to
written by shane
-
Assembly Of 454 Reads From Metagenomic
written by pmenzel •
-
Programs/Parameters For De Novo Transcriptome Assembly Of Ion Torrent Reads?
written by Ahdf-Lell-Kocks •
