Why figure from guppy basecaller show only 1 base in each event?
Hi, I am a beginner in nanopore. So I study about the guppy basecaller and try to use it. In normally from each peak in raw signal that represent current for each 5-mer. I wonder why in this picture that show the only 1 base for each peak instead show AACAT, CCGGT?
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briefly,it use middle base to represent 5mer, similarly in tombo resquiggle
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Probably because they wanted to keep the representation simple and are just showing the consensus calls on one strand.