Adding an extra/dummy chromosome to reference genome
What would be the recommended way to add a dummy/extra chromosome (in this case my vector/plasmid construct sequence) to a human reference genome? I am hoping to pull out reads aligned to this additional chromosome as well (also is there any caveat about me doing something like this?).
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Pierre Lindenbaum
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Omurice •
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Omurice •
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cat ref.fa plasmid.fa > newref.fa
samtools faidx newref.fa
samtools dict newref.fa > newref.dict
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Pierre Lindenbaum
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