Should I align sRNA-seq data to all ncRNA or ncRNA from a species?
Hi all, I have sRNA-seq data that I want to align with non-coding RNAs to extract tRNA, rRNA and other non-coding RNAs via bowtie. Do I have to align with ncRNAs from all plants or only my own species? Thanks in advance.
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If your sample is from a particular species then why would you want to align your data to all plants?