Primer Design - how to choose primer for SNPs in Primer 3
hello folks,
So, I want to design a Primer for SNPs in Primer3.
Should my Primers "flank" the SNP ? So, should I but my SNP within the sequence in " [ ] " ?
Or should I choose that Primers must overlab the junction, so put my SNP between "- -" ?
Thanks for any help
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