RT PCR Primer designing
Hello friends,
I would like to design RT PCR primers for few genes which is responsible for renal transplant (Pharmacogenomics) like cyp2c8, cyp3a5, cyp3a4, abcb1, TPMT, ugt1a9. I tried with Primer3 tool, but I don't know how to find mutation site in these genes.
i want to design primers for particular mutation site. plz help me how to find mutation site and design RT pcr primer.
Thank you in advance.
• 2,071 views
•
link
updated
by
Ram
•
written
by
preethisuga •
•
written
by
preethisuga •
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
Primer design scripts. OSError: Missing SEQUENCE tag
written by Young-Ho •
-
Adding flanking regions to a gene for primer design
written by eli_bayat •
-
Strain specific primers from multifasta file
written by rob •
-
Tool to design Q-PCR primers with exon skipping?
written by gabriel.monteiro233 •
-
Finding non-polymorphic region of genome for PCR primer design
written by joneill4x
-
QDD on Windows
written by kaiyang.lwl •
-
Housekeeper Genes and Real Time PCR
written by Maria.sewell •
-
Design primer for a family of bacterial genes
written by David
-
Design arbitrary degenerate primers (with non-binding criteria)
written by horea.christ •
-
design primer for alternative splicing events
written by pengchy
