We cannot possibly tell if something or what is wrong with your analysis or data without the data, parameters, and error messages produced. Please try to be much more precise or we cannot help you. Please edit and add the following info:

• The exact commandline used including all parameters, as text, no screenshots
• program version
• The exact and complete output and error messages, as text, not screenshots
• The input data, if too large, create an example input file as a gist on github