How to use eprimer3 command in linux?
Hallow, every one
when I use eprimer3 to generate primers for fasta sequence of sars cov 2 whole genome give Error:
Argument 'saracovII.fasta' : Too many parameters 3/2
what the best way to write eprimer3 command line to generate primers I used this command
eprimer3 -outfile=out.pr3 -sequence= saracovII.fasta
Thanks for your attention
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I dissolve the problem
EMBOSS_PRIMER32_CORE=/usr/bin/primer3_core eprimer32 -sequence=saracovII.fasta -outfile=out.pr3
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remove the blank after
sequence=after remove the blank get new error
Pick PCR primers and hybridization oligos Error: Missing SEQUENCE tag