Entering edit mode
2.3 years ago
Mo
▴
920
Hello Guys,
I did the following ways to get the annotation for each chip-seq data
1- Trim adaptors TrimGalore!
2- Aligned trimmed reads and sorts/converts to bam
3- Mark duplicates and filter reads
4- Quality metrics
5- Calculate cross-correlation
6- Call peaks
then I annotate each sample with ChIPseeker, so the input was the one
The problem is that I have now 6 annotated files and when I combine them using gene symbols (which has few per each start and end) then it gets more complicated. for instance, I may not be able to match exact start and end together for comparison
I would like to know if you have any strategy to combine such files? or will you even combine them before you do annotation?
Let me know your thoughts Thanks