Why does my ATAC trace only have 1 peak while everything is OK?
I strictly abide by the 10x genomics scATAC-seq protocol, and the quality of nuclei is very good. However, my data trace only have 1 peak at 200 bp.
I wonder if the transposon enzyme digested too much.
Thank you for your help!
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What are we talking about? Bioanalyzer/TapeStation, or alignment results?