More posts like this
-
GATK FilterMutectCalls
written by sk_24 •I am calling somatic variants using Mutect2 in paired mode. After variant calling, I applied FilterMutectCalls, followed by annotation using Funcotator. I wanted to ask …
-
PON and germline files for Mutect2 Tumor-only mode
written by Manuel •Hi! I am applying Mutect2 in tumor-only.mode having hg19 as reference and samples resulting of targeted sequencing (not only coding gene parts but also some …
-
Variant calling for matched samples
written by shivangi.agarwal800Hi Guys, I want to call variants for 36 samples having matched normal. Can you suggest me how to run (what are the steps) GATK …
-
Polyploidy found, and not supported by vcftools for a diploid data set.
written by Shripathi •Hi, I used gatk mutect2-select variant (retained only SNPs)-combinegvcfs to generate a vcf file for a diploid species. When I tried to process the vcf …
-
GATK Mutect2 errors during basic variant calling
written by michael.flower.14 •I've just installed GATK and am trying to do some basic variant calling. However when I try and run this line gatk Mutect2 -R $REF …
-
GATK Mutect2 tumor mode
written by cocchi.e89I am working on some human WGS tumor samples and I want to call somatic variants against the normal tissue sample. I see that Mutect2 …
-
Somatic variant calling with/without matched normal sample (Mutect2)
written by newbio17Hello, I'm currently testing GATK's tumor-only variant calling pipeline and came across an issue that I'm stuck on. I wanted to see how different the …
-
GATK4 (4.1.2.0) Mutect2 --germline-resource option
written by romagnoli.simone •Hi everybody, I am new analyzing WES and I try GATK4 workflow for the detection of somatic variants. I run Mutect2 in tumor-only mode with …
-
Can Mutect2 be used for paper?
written by Laven9 •It is said on the GATK web that Mutect2 is a BETA tool and is not yet ready for use in production. But I actually …
-
GATK4 Mutect2 variants IDs not shown
written by dganiewichHi everyone! I am a beginner using GATK, so bear with me please. I am trying to do my variant calling with GATK4 new Mutect2 …
what did you find so far ?
I have given this command for the generation of vcf file using Mutect2(GATK)
is this command correct please help!!!
You can find detailed help on how to use
mutect2here: https://gatk.broadinstitute.org/hc/en-us/articles/360035531132--How-to-Call-somatic-mutations-using-GATK4-Mutect2There is also a FAQ: https://gatk.broadinstitute.org/hc/en-us/articles/360050722212-FAQ-for-Mutect2
What is tumor.bam ? And ref.fasta is not accepting in gatk . And I have taken sam tool files as input to gatk Mutect2