Find Log2FC values from qPCR data

Hello!

I have got Fold Change (FC) values for a gene for 3 Biological Replicates.

1.Which is the correct method to calculate Log2FC ? Case1 or Case2 enter image description here ? Which case is incorrect and why?

If Case1 is correct? Using which column do i calculate the standard deviation/error?

I want to make a bar plot using Log2FC (Y axis) and Gene name (X axis) with error bars.

values log2fc rna-seq ct qpcr

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written 4.6 years ago by SKY •

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