How to identify differential enhancers?
Deseq2 or edgeR only allow the input of reads count to identify differential regions, but I get less differential enhancer with reads count, can I use the signal from the bigwig file as input to deseq2 or edgeR to identify the differential enhancer?
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No, the scores in bigwig files are not suitable for differential expression. The exact count information, so how many independent reads overlap a given region is lost. You have to create these counts from the BAM files.
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See the DiffBind or csaw documentation.
Ok, thank you very much!