This is a test version of Biostars. For the public version, visit https://www.biostars.org.
RiboZero RNA Seq vs Poly A RNA seq

Dear Community,

I wanted to know whether there is a difference in the downstream analysis of RiboZero RNA Seq data and Poly A+ RNA seq data?

I generally follow the pipeline shown below, is there something different that I need to do for RiboZero RNA seq data?

PIPELINE: FastQC > Adapter trimming > Alignment > feature Count (Count Matrix) > Differential Expression Analysis

Thank you

rna-seq

1 answer

In general, yes, you can use the same pipeline for differential expression analysis.

Log in to answer this question.