Its paired end
I will try fastp and let you know
Thank you!
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ashwin.koppayi •
Repost: Quality of RNA Seq Data
Hello All,
I am reposting the question for answer:
I am analyzing human RNA Seq data from a paper (PRJNA421274). I did FastQC on the RAW data and it showed high difference between %A and %T as shown in the link below
Even after trimming it is the same.Is it unsusal and should i use this samples? Is there a way to reduce this difference? I am new to RNA Seq Analysis.Any help is appreciated!
Thank you in advance.
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ashwin.koppayi •
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ashwin.koppayi •
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