Why am I getting numbers instead of gene names in Seurat slot data?
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
constructing gene-based pangenome from orthofinder output file
written by Bushra BibiHi all, I am constructing gene-based pangenome for fungus. I have used orthofinder. Please suggest which file should be used to find core and dispensible …
-
Is it possible to modify the gene names from the x-axis in Seurat::Dotplot?
written by ev97 •I have generated some dotplots for some genes in my scRNAseq data. As the `Seurat` object was already annotated with Ensembl IDs (and not gene …
-
Single cell analysis: Unable to subset cells in seurat object using desired nFeature_RNA cut off
written by TNF_alpha •```r #Violin plot to visualize QC matrices(before cell subsetting) VlnPlot(object = merged_seurat_objects, features = c("`nFeature_RNA`", "nCount_RNA", "percent_mt"), ncol = 3)![enter image description here][1] #Feature plots …
-
Monocle3 cell data set object (grabbing values from one column)
written by seattlescientist_01 •Hello! I am doing some single cell RNA seq analysis using Monocle3 software. Currently I have some Monocle objects in the form of cell data …
-
How to determine the total count for each gene in lymphotype B
written by dalibenam64 •Hello I really need help!! I don't speak English very well so sorry I have RDS object, this file is processed, normalized (using sct transform …
-
How to make scanpy read gene names to plot instead of read sequences
written by shome •I am trying to read a CSV file containing processed counts using the following command : ```r adata = sc.read_csv('GSM4885427_E18_JS-8_processed_counts.csv') ``` It results in AnnData …
-
Pearson Correlation Single cell seurat
written by PayalHello, I am doing my singlecell analysis in Seurat. I want to create Pearson correlation matrix of a set of genes per cluster basis. I …
-
Seurat subset cluster cell annotation
written by leranwangcs •Hi, I used Seurat to do clustering, then singleR to do cell annotation. In this annotation, I got multiple cell types such as B cells …
-
Cell ranger reads do not match Seurat reads
written by firestarThis is the summary from the CellRanger run showing ~150,000 reads per cell. ![cellranger-report][1] But, when I read the results into R, I get only …
-
Defining the threshold for reads and features
written by zizigoluI have some Indrop scRNA-seq The number of genes per cell and number of read seems like this ![enter image description here][1] ![enter image description …
In your counts table you set the first column to null, and commented out the line before that may have set the rownames to gene names. After loading the count matrix and before setting
V1toNULL, what does the count matrix look like?If the gene names are in the first column you need to uncomment the line that has
rownames(counts) <- counts$V1and run it before you remove that column.Thank you, I had commented that line out since it was 1.3 GB of data and it was taking a while on my humble laptop :)
It is working now.