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Can the Non strand specify RNAseq data annotate genome?

Hi everyone,

I have many rnaseq samples data, but I find those data are not strand specific. I read many paper and I found they annotated their genome using the strand specific RNAseq data. Can I use my non-strand specify RNAseq data to annotate gene structure of genome?Thank you very much.

rna-seq
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written by yiren • yiren

1 answer

Yes sure you can, it is just most of time more accurate to perform annotation using stranded library RNAseq data.

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written by Juke34 Juke34

thank you very much.

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written by yiren • yiren

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