Thank you very much !! I didn't pay attention to this detail... Now it worked!!
VCFtools doesn't keep any variants using GATK output
Hi guys!
I'm having trouble using vcftools to filter snp through GATK output.
For information, I used the command HaplotypeCaller to make SNP-calling of 12 samples; I used the CombineGVCFs to join the 12 VCFS and make the joint call after merging the vcf files; I used the VariantFiltration for a initial filtration.
Now, I want to make a filtration using VCFtools, for this, I used the command :
vcftools --vcf output_gatk.vcf --remove-indels --recode --recode-INFO-all --out SNPs_gatk_only
But, after the filter, the VCFtools doesn't keep any variant, like this:
After filtering, kept 12 out of 12 Individuals
Outputting VCF file...
After filtering, kept 0 out of a possible 68599004 Sites
No data left for analysis!
Run Time = 438.00 seconds
Am I doing something wrong? My VCF input has a header like this:
#CHROM POS ID REF ALT QUAL FILTER INFO FORMAT T1R2-6H T1R3-24H T1R3-6H T1R6-24H T1R7-24H ...
LG1 1 . G <NON_REF> . PASS END=14 GT:DP:GQ:MIN_DP:PL ./.:0:0:0:0,0,0 ./.:0:0:0:0,0,0 ./.:0:0:0:0,0,0 ...
LG1 15 . A G,<NON_REF . PASS END=31 GT:DP:GQ:MIN_DP:PL ./.:0:0:0:0,0,0 ./.:0:0:0:0,0,0 ./.:1:3:1:0,3,19 ...
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1 answer
you're working with a GVCF file. A gvcf should be first post-processed with "gatk GenotypeGVCFs"
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