your answer saved my time. Thank you!
Hello all,
I am using maker2 to annotate the genome. For gene prediction, I am trying to use snap-hmm. While training snap, to generate parameters file I used the command
forge export.ann export.dna
and I received the following error:
ZOE ERROR (from forge): impossible error7
ZOE library version 2013-02-16
I can't figure out what this error means. I couldn't find anything in maker-devel discussion group either. If anyone can help me, I greatly appreciate the help.
Thanks very much and have a great weekend!
1 answer
I contacted the author and here is the work around for this problem. In short, I have to follow these commands to reach the error (forge step)
$ gff3_merge -d genome_master_datastore_index.log -g
$ mkdir snap_training
$ mv genome.all.gff ./snap_training/
$ cd snap_training/
$ maker2zff -n genome.all.gff
$ fathom -validate genome.ann genome.dna
# prints to stdout after validation, several pages long.
$ fathom -gene-stats genome.ann genome.dna
1365 sequences
0.413186 avg GC fraction (min=0.339882 max=0.666334)
24265 genes (plus=12222 minus=12043)
4313 (0.177746) single-exon
19952 (0.822254) multi-exon
181.480927 mean exon (min=1 max=11937)
792.193176 mean intron (min=30 max=48985)
$ fathom -categorize 1000 genome.ann genome.dna
$ fathom -export 1000 uni.ann uni.dna
$ mkdir parameters
$ cd parameters
$ forge ../export.ann ../export.dna
ZOE ERROR (from forge): impossible error7
ZOE library version 2013-02-16
If your models have a lot of errors, then the step fathom -export 1000 uni.ann uni.dna will ignore only upto 1000 errors. To ignore more that that, you need to specify the option -export 1000 -plus. this will solve the problem. So use:
$ fathom -export 1000 -plus uni.ann uni.dna
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