scRNA-seq data analysis - R
Hi! Could anyone suggest some tutorial how to do scRNA-seq data analysis in R given files:
- .tsv with barcodes
- .tsv with features
- and .mtx matrix
I am new in this and I will appreciate any help.
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2 answers
A simple Google search will give you many resources to explore.
- http://bioinf.wehi.edu.au/edgeR/10XWorkshop/10X.pdf
- http://bioinf.wehi.edu.au/edgeR/10XWorkshop/
- https://www.singlecellcourse.org/
- https://github.com/bishwaG/Single-cell-RNA-seq-data-analysis-2018
- https://satijalab.org/seurat/articles/get_started.html
- https://www.nature.com/articles/s41596-020-00409-w
- https://link.springer.com/protocol/10.1007%2F978-1-0716-1307-8_19
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The best resource: https://bioconductor.org/books/release/OSCA/
Please google for loading mtx into R (hint, it is the readMM function from the Matrix package).
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