Demultiplex Quality
Hi all,
I'm working on a project that requires to demultiplex the sequencing data twice (for example I have 400 samples, the first time I will demultiplex to 4x100 samples, and the second time I will demultiplex to individual file). I'm wondering if I should just demultiplex once and avoid any potential loss of reads? Will the quality of the data be lower if I demultiplex twice?
Thank you!
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There is not enough information in your post as it stands. What are you demultiplexing using? Internal barcodes/Illumina indexes? Why should there be any potential loss of reads? Why should the quality be lower (assuming you are referring to quality scores of each base)?