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GO Term analysis

I have ~450 genomic regions of 250kb each in hg38.

I wanted to do GO term analysis of genes in these regions.

In python, I used gencode.v38.basic.annotation.gff3.gz filtered on feature='gene'. I get ~30000 genes.

Using Panther Classification System, I get ~8000 hits.

If I use the UCSC genome browser, with track NCBI Refseq track and Refseq(all) table, I get only ~1800 genes.

What is the best way to extract genes for GO term analysis?

go

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