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Can you predict the size of a BAM file based on the fasta?

I'm apply for computing resources on a cluster that asks for an estimate of storage space. So far I only have compressed fastq files, making it hard to judge how much total space I will need. Is there any rule of thumb for estimating the total size of the BAM/SAM files based on the size of the fastq?

storage mapping bam
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updated by Kevin Blighe Kevin Blighe • written by James Reeve James Reeve

Within a ball park. It will depend to a large extent on amount of secondary alignments and/or if you are going to keep unmapped fastq reads in the BAM file.

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written by GenoMax GenoMax

I guess it would depend on a lot of factors. For my purposes I won't be keeping either secondary alignments or unmapped reads. Hopefully, this helps a bit in coming up with a rough estimate of storage.

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written by James Reeve James Reeve

'Machine learning' could sample reads from a FASTQ file, and then predict the size of the aligned BAM file, for sure

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written by Kevin Blighe Kevin Blighe

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