Query regarding WGCNA module formation
I want to form a WGCNA module, I took the genes which are expressed highly according to their standard deviation of my desired sample group, I want to ask is their need to normalize the gene count series matrix again before doing the experiment?
Please tell me there is a total of 9 samples and I choose highly expressed 4000 genes in 9 samples. What type of normalized procedure is needed?
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andres.firrincieli
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microorganism_001 •
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microorganism_001 •
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Don't bother using WGCNA if you have just 9 samples. WGCNA authors suggests > 20 samples, while 15 is the minimum number of samples (link)
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