Hi,

I am using InterProScan-5.51-85.0 to annotate ~20K proteins from a Poceae plant. I use for it the next command:

/data/software/interproscan-5.51-85.0/interproscan.sh \
    --output-dir /data/work/poceae/annotation_result \
    --input /data/triticum/genomes/poceae/poceae_annotation_200916/annotationInterPROScan/poceaev2.1_annotation_200916_HC_pep.valid.00.fasta \
    --appl PFAM,PANTHER \
    --formats TSV \
    --iprlookup \
    --goterms \
    --pathways \
    --seqtype p

The computer is behind a firewall so I need a proxy to reach outside. To do that, I have modified the file interproscan.properties as follows:

#proxy set up
precalculated.match.lookup.service.proxy.host=proxy.satd.uma.es
precalculated.match.lookup.service.proxy.port=3128

In addition, in the same session of Linux, I have exported the corresponding proxy variables:

export HTTP_PROXY=http://proxy.satd.uma.es:3128 
export HTTPS_PROXY=http://proxy.satd.uma.es:3128 
export FTP_PROXY=http://proxy.satd.uma.es:3128

Unfortunately, no matter how many proteins contains the fasta file, I obtain the next messages again and again:

2021-05-06 12:22:01,980 [Thread-10] [uk.ac.ebi.interpro.scan.precalc.client.MatchHttpClient:142] WARN - HTTP Proxy Warning: this run is configured to use an http proxy, InterProscan is however unable to handle this properly.

2021-05-06 12:22:01,980 [Thread-6] [uk.ac.ebi.interpro.scan.precalc.client.MatchHttpClient:142] WARN - HTTP Proxy Warning: this run is configured to use an http proxy, InterProscan is however unable to handle this properly.

Though it is a Warning and not an Error, it is clear that the lookup is not working smoothly. Is the program correctly connecting to the lookup service? Am I obtaining a good annotation for each of my proteins? What is the actual problem and how to remove it?

Thank you in advance!