RNAseq Per tile sequence quality question
Hi I have a question about the Per tile sequence quality from a fastqc report. About half my samples have this signature, the other have are blank (all blue). Is this something to be concerned about? Everything else looks fine. This is the report after trimming with TrimGalore! Thanks for taking a look
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cook.675 •
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Since you have pretty long reads (150 bp) I would say this should cause no concern (quality degrading towards end of run). If alignments look fine then you should go on to the next steps of analysis.
Thank you for your reply