GC content of WES cohort
Hello,
I know a lot of questions have been asked before about GC content, but I just wanted to see if anyone had experience with WES GC content. I have 134 WES (not all the same exome panel or from the same sequencing company).
From reading the FASTQC manual I can see that if it is more than 15% it may be caused by bias?
Does anyone have any experience of this, and if so does my data look okay? This was made on FASTQC and then combined using multiqc. I will attach a before and after from cutadapt.
Before trimming:
After trimming:
Thanks!
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