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Difference between trim galore report and Fastqc pre trimming

Hi everyone !

I have a small question about the trim galore report.

I ran my command on 100bp paired RNA-seq data :

trim_galore --dont_gzip --stringency 1 --fastqc --length 15 --paired -o [output] [input_R1] [input_R2]

The report gives me this information :

R1 :

Total reads processed:              28,073,466
Reads with adapters:                17,138,946 (61.1%)
Reads written (passing filters):    28,073,466 (100.0%)

R2 :

Total reads processed:              28,073,466
Reads with adapters:                17,165,013 (61.1%)
Reads written (passing filters):    28,073,466 (100.0%)

But the problem is that in my fastqc report I don't have as many adapters, a little over 30% for R1 and for R2.

R1

R2

Does trim galore sum R1 and R2 in its report?

Thank you very much for your help!

trim-galore

1 answer

I think Trim Galore reports the total adapters percentage, but fastqc reports the adapters percentage based on its location in reads.

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