Differential expression analysis with LIMMA results
Hello,
I did a differential expression analysis of RNA seq data (normalized RSEM) from TCGA with VOOM and another time with Limma trend , but i got as result most of the genes with positive log2 fold change and significant p-value. Do you have any idea about this issue ?
this is the figure of my volcano plot :
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thanks for the reply , so for the groups : we have 2 groups high and low of a gene and samples are patients.
I don't have an MA-plot i just did a volcano plot.
this an example of the results tab