About combining the forward and reverse sequence
I have a sanger sequence of reverse and forward reads, but when i trimmed in geneious software it showed no contig found. This no contig found implies that there is no overlap of forward and reverse sequence. Does that mean there is an error in sanger sequencing or can we do anything about such reads.
• 3,229 views
•
link
updated
by
GenoMax
•
written
by
ruddhida •
•
written
by
ruddhida •
1 answer
You can also try to play with one pair of sequences manually, since one of the sequences would have to be reverse complement to the other.
You can try pasting one sequence here and see if you can find any overlap.
• 0 views
•
link
written
by
lmlukoseviciute •
Log in to answer this question.
More posts like this
-
Bamutils/ClipOverlap
written by chrisk •
-
Identification of presence of genes by aligning the illumina reads to reference genome with Geneious
written by rthapa •
-
Total number of trimmed reads less than number of mapped reads
written by kspata
-
Sanger sequencing Result: Difference between forward and reverse DNA strands
written by shuvo.bgenstu •
-
False heterozygosity at chromosome X for male sample in Exome Sequencing
written by koay •
-
Merge paired reads for error-correction
written by Mick •
-
Adapter Removal - Nextera LIbrary
written by ailinleti •
-
Any suggestion for sanger sequencing result editing
written by liaoyunshi •
-
Ho to fix a VCFAnnotator software error
written by genome21 •
-
WGS analysis suggestions
written by madhubhaniesenadheera •
show the read pair here, it would be hard to comment on what one might see not knowing what the data is what has been done to it
Reasonably certain
geneioususes tools from BBMap suite to do some of these operations. You should merge the reads first before checking if they have contaminants.