That is correct.
Hi all,
I am generating a million paired end reads with wgsim using the following command
wgsim -N 1000000 -1 100 -2 100 hg19.fa read1.fq read2.fq
The output files are generated fine. The screen output seems to be some sort of mutation report but I can't seem to spot the correlation between this and the content of my output files.Am I totally misinterpreting something?
3 answers
As far as I remember the output to stdout is a SAM pileup file including the positions of the mutations. http://samtools.sourceforge.net/pileup.shtml
The stdout looks like this
chrX 89985560 T C -
chrX 89990640 T Y +
chrX 89991017 C M +
chrX 89991096 G R +
chrX 89994004 A R +
chrX 89994987 A W +
chrX 89995790 A R +
Even when I generate 100 reads the stdout is 2,923,254 lines long. So is is just generating and outputting mutations for the whole genome and then generating reads indepently of these across the genome?
So where do I find out what the 'W's and 'R's etc mean? And also, what is the quickest way of finding out which of these mutations were incorporated into the actual reads I generated?
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