Illumina Read Quality
Hi all,
I am new to NGS and i have a very naive question.
Illumina reports the score of each base in the read as a phred score. But, how is the total read quality calculated?Is it the sum of scores of all the bases in the read? Any comments are valuable.
1 answer
There are a lot questions here focused on read quality so your question is redundant -- you can find other questions relating to your topic by using the custom search.
I would also recommend this page -- FASTQ format and read quality -- to provide you some background on how the Phred quality score is calculated and what each character means.
Log in to answer this question.
More posts like this
-
how shoud the phred score be intepreted?
written by QX •
-
Obtain phred scores for each read
written by anuradharavi10 •
-
PHRED scores and sequence errors
written by Dunois •
-
deML 'rgqual' parameter
written by ovon •
-
Should I use reads with good quality but failed-vendor flag?
written by Tao
-
Phred Score/Base quality score in Pysam
written by Lylthera •
-
How are expected error rates calculated?
written by Andrew •
-
Combining quality scores when aligning/assembling
written by earonesty
-
Comparing Quality Of Read 1 And Read2 Of Illumina Pe
written by Juliofdiaz
-
Read To Consensus Quality Scores
written by Lee Katz
Similar one was discussed here