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Ava: No Sample/Reference Data To Align

We have received .sff files from a GS FLX 454 Titanium run of a single forward sequence amplicon. I have followed the AVA manual to create a reference, sample, amplicon, etc. and have made the necessary associations, and loaded in the SFF file. Blueprints, MIDs, etc. are all shown. The relevant "Active" entry is selected

However, after computing the results, I am always shown "No sample/reference data to align"" and "No variants to load". I have tried with a known variant, and without any variants specified. I have also tried with different blueprints selected.

This thread was not helpful and there have been no replies to my thread here.

Suggestions, please?

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updated by Biostar Biostar • written by Sabrewolfy • Sabrewolfy

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