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Reading Illumina Final Report Using Beadarraysnp R Package

Hi,

I have created a report file from my genotyping data in Genome Studio. I was trying to use the R package "beadarraySNP"

This is the error that I got 

**"Error in read.SnpSetIllumina("finalreport.txt",  : 
Sample sheet error, column(s) Sample_Name, Sentrix_Position, Sample_Plate, Pool_ID, Sentrix_ID are not available"**

Infact I tried using "Nochecks=TRUE. It doesn't seem to work. Any help/suggestion is appreciated.

Thanks

r illumina bioconductor genotyping snp
• 5,785 views • link
written by venks venks

can you post the first lines of the file "finalreport.txt"? Otherwise, it's difficult for us to understand what is going wrong.

• 0 views • link
written by Giovanni M Dall'Olio Giovanni M Dall'Olio

3 answers

Based on page 28 of the documentation, it looks like you might be specifying the final report file where you really should be specifying the sample sheet. I think you need to do something like:

SNPdata <- read.SnpSetIllumina("samplesheet.csv", reportfile="final report.txt")

Where both files reside in the current directory.

• 196 views • link
written by Matt Shirley Matt Shirley

Thanks Matt. I tried inputting the sample Sheet through a data frame. It shows the "missing column error for sample sheet". I exported the report from Genome studio. I do not know why the Sample_map file wasn't able to generate all the required columns.

• 89 views • link
written by venks venks

Based on the documentation, I think you are supposed to give a string containing the file name of the sample sheet, not the actual data. You might be over-thinking this :)

• 0 views • link
written by Matt Shirley Matt Shirley

I tried the same ..

Error in read.SnpSetIllumina(samplesheet = "C:\Users\gen\Sample_Map.txt", : Cannot find "[Data]" in samplesheet file

I ended up getting this error even if I have the data in the samplesheet.I am still not sure if I ended up messing up the data or the program..

Thanks again.

• 122 views • link
written by venks venks

When you have also saved the sample_map while saving the final report in Genome Studio you can do

SNPdata<- read.SnpSetIllumina(Sample_Map2Samplesheet("sample_map.txt"),reportfile="finalReport.txt",nochecks=TRUE)
• 117 views • link
written by Jan Oosting Jan Oosting

SNPdata<- read.SnpSetIllumina(Sample_Map2Samplesheet("sample_map.txt"),reportfile="finalReport.txt",nochecks=TRUE)

Blockquote

Sample_Map2Samplesheet - creates a samplesheet

Blockquote

Nochecks= TRUE when we don't have all the fields in the final report

• 90 views • link
written by venks venks

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